目录号： #9001S,#9001M
(q)PCR,Genotyping
| 产品名称 | HiDi® DNA polymerase |
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| 目录号 | #9001S,#9001M |
| 描述 | HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® DNA polymerase is a highly selective aptamer-based fast-start formulated DNA polymerase variant suitable for SYBR chemistry, specially optimized for assays in which high discrimination is required. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies. For more information, please check : |
| 应用 | (q)PCR,Genotyping, 若需了解更多应用，请联系我们 |
| 组分/含量 | S pack: 250 U, 5 U/µl, 1 x 50 µl HiDi® DNA polymerase; 1 x 1.25 ml 10x HiDi reaction buffer M pack: 250 U, 5 U/µl, 1 x 200 µl HiDi® DNA polymerase; 2 x 1.25 ml 10x HiDi reaction buffer |
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| 储存条件 | -20°C |
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| 运输条件 | Cold packs |
| 库存及货期 | 确认合同后4周左右，请与客户经理联系确认 |
| 文献 | Drum, M., Kranaster, R., Ewald, C., Blasczyk, R., & Marx, A. (2014). Variants of a Thermus aquaticus DNA polymerase with increased selectivity for applications in allele-and methylation-specific amplification. PloS one, 9(5), e96640. Barraud, N., Létoffé, S., Beloin, C., Vinh, J., Chiappetta, G., & Ghigo, J. M. (2021). Lifestyle-specific S-nitrosylation of protein cysteine thiols regulates Escherichia coli biofilm formation and resistance to oxidative stress. npj Biofilms and Microbiomes, 7(1), 1-11. Miotto, O., Sekihara, M., Tachibana, S. I., Yamauchi, M., Pearson, R. D., Amato, R., ... & Mita, T. (2020). Emergence of artemisinin-resistant Plasmodium falciparum with kelch13 C580Y mutations on the island of New Guinea. PLoS pathogens, 16(12), e1009133. Yang, Z., Le, J. T., Hutter, D., Bradley, K. M., Overton, B. R., McLendon, C., & Benner, S. A. (2020). Eliminating primer dimers and improving SNP detection using self-avoiding molecular recognition systems. Biology Methods and Protocols, 5(1), bpaa004. Morisaka, H., Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., ... & Mashimo, T. (2019). CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Nature communications, 10(1), 1-13. Madhuri, S., Bártulos, C. R., Serif, M., Lepetit, B., & Kroth, P. G. (2019). A strategy to complement PtAUREO1a in TALEN knockout strains of Phaeodactylum tricornutum. Algal Research, 39, 101469. |
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