目录号: #9201S,#9201M
(q)PCR,Genotyping
产品名称 | HiDi® Taq DNA polymerase |
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目录号 | #9201S,#9201M |
描述 | HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® Taq DNA polymerase is a highly selective aptamer-based fast-start formulated DNA polymerase variant, specially optimized for assays in which high discrimination is required. HiDi® Taq DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. HiDi® Taq variant has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs. By using HiDi® DNA Taq polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies. For more information, please check : |
应用 | (q)PCR,Genotyping, 若需了解更多应用,请联系我们 |
组分/含量 | S pack: 250 U, 5 U/µl, 1 x 50 µl HiDi® Taq DNA polymerase; 1 x 1.25 ml 10x HiDi reaction buffer M pack: 250 U, 5 U/µl, 1 x 200 µl HiDi® Taq DNA polymerase; 2 x 1.25 ml 10x HiDi reaction buffer |
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储存条件 | -20°C |
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运输条件 | Cold packs |
库存及货期 | 小包装样品现货 |
文献 | Drum, M., Kranaster, R., Ewald, C., Blasczyk, R., & Marx, A. (2014). Variants of a Thermus aquaticus DNA polymerase with increased selectivity for applications in allele-and methylation-specific amplification. PloS one, 9(5), e96640.
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