分子诊断原料

MedixMDx Fast Taq Polymerase is a thermostable recombinant DNA polymerase expressed by Thermus aquaticus. MedixMDx Fast Taq Polymerase has 5' to 3' polymerase activity and 5' to 3' exonuclease activity, but lacks 3' to 5' exonuclease activity. The enzyme uses state-of-the-art polymerase technologies and optimized buffer chemistry for improved polymerase processivity, yield, sensitivity, and specificity. MedixMDx Fast Taq Polymerase has an error rate of approximately 1 error per 2.0 x 10^5 nucleotides incorporated.

MedixMDx HS Taq DNA Polymerase is a hot-start thermostable recombinant DNA polymerase expressed by Thermus aquaticus. MedixMDx HS Taq Polymerase has 5' to 3' polymerase activity and 5' to 3' exonuclease activity, but lacks 3' to 5' exonuclease activity. The enzyme uses state-of-the-art hot-start antibody-based technology as well as optimized buffer chemistry for high sensitivity and yield, and rapid polymerase processivity. The enzyme is ideal for complex, difficult DNA templates and is resistant to PCR inhibitors. MedixMDx HS Taq DNA Polymerase has an error rate of approximately 1 error per 2.0 x 10^5 nucleotides incorporated.

MedixMDx HS HiFi DNA Polymerase is an aptamer-based hot-start, high fidelity hyperthermophilic recombinant DNA polymerase from the archaeon Pyrococcus furiosus. MedixMDx HS HiFi DNA Polymerase exhibits 5' to 3' polymerase activity and 3' to 5' exonuclease activity. The optimized buffer chemistry facilitates high sensitivity, yield, specificity, robust and rapid polymerase processivity. The enzyme is ideal for long, complex, difficult DNA templates and is resistant to PCR inhibitors. MedixMDx HS HiFi DNA Polymerase has a lower error rate than standard Taq DNA polymerase (100x) and therefore, is suitable for PCR applications where higher accuracy is needed.

MedixMDx HighScript Reverse Transcriptase is a modified version of MMLV reverse transcriptase with noticeable thermostability and high enzymatic activity. This enzyme is offered as a blend with an RNase inhibitor to prevent RNA degradation. MedixMDx HighScript Reverse Transcriptase together with enhanced buffer chemistry enables fast, efficient and accurate synthesis of the cDNA molecule.

MedixMDx RNase Inhibitor is a protein that blocks the activity of a variety of ribonucleases due to very fast non-covalent binding of the inhibitor to the active sites of enzymes. Medix RNase Inhibitor is resistant to oxidative stress due to its lack of cysteine residues and it remains active at 65°C for at least 30 minutes. This RNase inhibitor blocks a wide range of RNases, but does not inhibit RNases T1, T2, U1, U2, CL3, RNase I and H.

MedixMDx Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activitiy, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. MedixMDx Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C.

MedixMDx Fast Bst Polymerase is a recombinant DNA polymerase expressed by Geobacillus stearothermophilus. The DNA polymerase displays high strand displacement activity, exhibits 5’ to 3’ polymerase activity, but lacks 5’ to 3’ exonuclease activity. MedixMDx Fast Bst Polymerase is tolerant to inhibitors, enabling rapid and robust isothermal nucleic acid amplification reactions at a constant temperature. The typical reaction temperature is 65°C. Addition of an intercalating dye allows the reaction to be monitored using a real-time PCR instrument.

Taq hotstart DNA polymerase is an aptamer-based fast-start formulated DNA polymerase supplied with a 10x reaction buffer that has been specially designed for optimal PCR performance and DNA polymerase activity. This DNA polymerase is suitable for a wide range of PCR applications. Taq hotstart DNA polymerase has a 5'-3'-exonuclease activity and therefore, can be used for hydrolysis probe-based real-time PCRs.

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Taq hotstart DNA polymerase

RevTaq RT-PCR DNA polymerase is an engineered, extremely thermostable, aptamer-based fast-formulated reverse transcriptase and combined DNA polymerase with a half-life at 95°C of > 40 min. This enzyme allows reverse transcription reactions at high temperatures, minimizing the problems encountered with strong secondary structures in RNA. RevTaq RT-PCR DNA polymerase allows “zero-step” RT-PCRs directly from RNA templates without an isothermal reverse transcription step, as reverse transcription takes place simultaneously with DNA amplification during the cycled PCR elongation step. RevTaq RT-PCR DNA polymerase is the pure, reverse transcription active enzyme ingredient of our Volcano3G® RT-PCR Master Mixes.

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RevTaq RT-PCR DNA polymerase, 50x concentrated glycerol stock

HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. HiDi® DNA polymerase is a highly selective aptamer-based fast-start formulated DNA polymerase variant suitable for SYBR chemistry, specially optimized for assays in which high discrimination is required. HiDi® DNA polymerase efficiently amplifies from primers that are matched at the 3'-end and discriminates primers that are mismatched. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of > 10^4 wild-type copies.

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HiDi® DNA polymerase